TABLE 2.

List of nucleic acid extraction methods suitable for POC analysis.

Extraction method	Advantages	Disadvantages	Applied for plant pathogens	References
Crude extract	Fast, inexpensive, no need of trained personnel	Presence of contaminants, lack of sensitivity and reproducibility	Yes	Miles et al. (2015); Minguzzi et al. (2016); Getaz et al. (2017); Munawar et al. (2019)
Magnetic beads	Reliable, high-quality genetic material, no need of centrifugation	Expensive, not completely user friendly	Yes	Chang et al. (2013); Schwenkbier et al. (2015b); Lin et al. (2015); DeShields et al. (2018); Wei et al. (2018); Lei et al. (2019)
Piercing methods	Fast, inexpensive, no need of trained personnel	Lack of sensitivity, necessity of optimization	Yes	Fukuta et al. (2005); Suzuki et al. (2016); Paul et al. (2019, 2020); Wilisiani et al. (2019)
Cellulose filter paper	Fast, inexpensive, no need of trained personnel	Possible absorption of inhibitors, necessity of optimization	Yes	Lu et al. (2016); Zou et al. (2017)