TABLE 3.

List of the main polymerase chain reaction (PCR)-based amplification methods applied for POC plant pathogen detection.

Method	Target	Advantages	Disadvantages	References
Conventional PCR	DNA/RNA	Reliable, relatively cheap	Time consuming, requires a thermocycler	Julich et al. (2011); Schwenkbier et al. (2014)
Nested-PCR	DNA/RNA	Very specific	Expensive, time consuming, requires a thermocycler	Costa et al. (2012); Wei et al. (2018)
Real-time PCR	DNA/RNA	Allows absolute quantification of DNA; no need of post-amplification detection	Time consuming, requires highly purified genetic material, requires a thermocycler	Tomlinson et al. (2005); Arif et al. (2013); Koo et al. (2013); Baidoo et al. (2017); Bini et al. (2018); DeShields et al. (2018); Fotiou et al. (2019)
Digital PCR (ddPCR)	DNA/RNA	High sensitivity, resilient to contaminants, allows absolute quantification of DNA	Expensive, requires specific equipment	Selvaraj et al. (2018); Voegel and Nelson (2018); Zhong et al. (2018); Liu et al. (2019)