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. 2020 Oct 28;8:573781. doi: 10.3389/fcell.2020.573781

FIGURE 2.

FIGURE 2

Growth differentiation factor 8 (GDF8) upregulates the expression of follistatin-like 3 (FSTL3) in HTR8/SVneo and primary EVT cells. A,C,E, and G: HTR8/SVneo (A,C) or primary EVT (E,G) cells were treated for 24 h with the vehicle control (Ctrl) or different concentrations (10, 25, or 50 ng/mL) of GDF8; the mRNA (A,E) and accumulated (C,G) levels of FSTL3 were examined using RT-qPCR and ELISA, respectively. B, D, F, and H: HTR8/SVneo (B,D) or primary EVT (F,H) cells were treated with the vehicle control (Ctrl) or 25 ng/mL GDF8 for 3, 6, 12, 24, or 48 h; the mRNA (B,F) and accumulated (D,H) levels of FSTL3 were examined using RT-qPCR and ELISA, respectively. The results are displayed as the mean ± SEM of at least three independent experiments, and the values with different letters are significantly different (P < 0.05).