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. 2020 Oct 28;8:573781. doi: 10.3389/fcell.2020.573781

FIGURE 3.

FIGURE 3

Follistatin-like 3 (FSTL3) mediates growth differentiation factor 8 (GDF8)-induced cell invasion in HTR8/SVneo and primary EVT cells. A,B,D, and E: HTR8/SVneo (A,B) or primary EVT (D,E) cells were transfected for 24 h with 20 nM non-targeting control siRNAs (siCtrl) or 20 nM siRNAs targeting FSTL3 (siFSTL3), after which the cells were treated with the vehicle control (Ctrl) or 25 ng/mL GDF8 for 24 h; the mRNA (A,D) and accumulated (B,E) levels of FSTL3 were examined using RT-qPCR and ELISA, respectively. C,F: HTR8/SVneo (C) or primary EVT (F) cells were transfected for 24 h with 20 nM siCtrl or 20 nM siFSTL3, after which the cells were treated with Ctrl or 25 ng/mL GDF8 for 24 h; cell invasion was examined using the Matrigel-coated transwell assay. The results are displayed as the mean ± SEM of at least three independent experiments, and the values with different letters are significantly different (P < 0.05).