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. 2020 Oct 28;10:570733. doi: 10.3389/fonc.2020.570733

Figure 5.

Figure 5

miR-550a-5p accelerated LUAD cell proliferation. (A) miR-550a-5p level in 29 LUAD tissues and paired adjacent tissues investigated by RT-qPCR. (B) miR-550a-5p level in normal lung epithelial cell line 16HBE and five LUAD cell lines investigated by RT-qPCR; U6 was used as an internal control. (C,D) Verification of miR-550a-5p expression in H1299 and A549 cell lines after lentivirus treatment by RT-qPCR. (E,F) CCK-8 was used to determine the proliferation of transfected H1299 and A549 cell lines. (G,H) Effect of miR-550a-5p on colony-forming capacity of H1299 and A549 cell lines treated with lentivirus. (I,J) Representative profile of EdU cell growth in H1299 and A549 cell lines after transfection with lentivirus, respectively, compared with the control. Data expressed as mean ± SD (*P < 0.05; **P < 0.01; ***P < 0.001).