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. 2020 Nov 10;10:19396. doi: 10.1038/s41598-020-76538-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of the Dux family, Zfp352, and Zscan4d during preimplantation development. Fifty MII stage oocytes and embryos at the 1-, 2-(early, mid and late), 4-cell, and morula stages were collected at 13, 16, 24, 32, 40 and 70 h post insemination (hpi), respectively, and subjected to reverse transcription polymerase chain reaction (RT-PCR). Two independent experiments were conducted and similar results were obtained. (A) Electrophoresis images of the Dux family, Zfp352, Zscan4d, and rabbit α-globin (external control) PCR products. (B) The band densities in (A) were quantified using ImageJ. The band densities of the three genes are relative to that of rabbit α-globin. The values at the early 2-cell stage were set to 1 and the relative values were calculated. The average value from two experiments is shown.