Figure 4.

Effect of overexpression of the Dux family on the development of 2-cell stage embryos. A blastomere of a 2-cell stage embryo was microinjected with cRNA encoding DuxG1 or Dux G9 and then cultured until 40 hpi in vitro. Control embryos were not injected. (A,B) Schematic diagrams of the experiments. Photograph of embryos that were injected with (A) or without (B) DuxG1 or DuxG9 cRNA. Scale bar = 100 μm. (C) The developmental stages of the embryos at 40 hpi. Parentheses indicate the number of embryos observed at 40 hpi. (D) DuxG1 and RFP cRNA were simultaneously microinjected into a blastomere of 2-cell stage embryos and cultured until 45 hpi in vitro. Then the embryos were immunostained with RFP antibody at 45 hpi. DNA was detected by DAPI staining. Scale bar = 20 μm.