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. 2020 Nov 10;10:19393. doi: 10.1038/s41598-020-76483-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Accumulation of ROS in control and bacterized-plantlets after challenge with B. cinerea. (a) H₂O₂ content in control and bacteria-treated grapevine plantlets at 8hpi. Different letters indicate significant differences (P ≤ 0.05). (b,c) the histochemical detection of H₂O₂ and O₂^- in leaves of control and bacterized plantlets 8 hpi. PBS grapevine plantlets inoculated with PBS, Bc grapevine plantlets inoculated with PBS and infected by Botrytis, BE17 and BE24 plantlets inoculated with strain BE17 or BE24, Bc + BE17 and Bc + BE24 plants inoculated with strain BE17 or BE24 and infected by Botrytis. Values shown are means ± SD of three independent repetitions (each repetition was realized in triplicate). Bars = 5 mm.