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. 2020 Nov 10;10:19393. doi: 10.1038/s41598-020-76483-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The expression of several defense markers in control and bacterized plantlets at 24 h post infection with Botrytis. Transcript accumulation of defense-related-genes, including pathogenesis related genes PR5, PR10, class IV chitinase (Chit4C), jasmonate zim-domain (JAZ) and glutathione S-transferase (GST) was carried out by qRT-PCR 24 h post challenge with the pathogen. The expression levels were normalized using two housekeeping genes [elongation factor 1-α (EF1a) and 60S ribosomal protein L18 (60RSP)] as internal controls. Results are expressed as the fold increase in transcript level compared to non-bacterized plantlets treated with PBS. PBS grapevine plantlets inoculated with PBS, Bc grapevine plantlets inoculated with PBS and infected by Botrytis, BE17 and BE24 grapevine plantlets inoculated with strain BE17 or BE24, Bc + BE17 and Bc + BE24 grapevine plantlets inoculated with strain BE17 or BE24 and infected by Botrytis. Values shown are means ± SD of three independent repetitions (each repetition was realized in triplicate). Different letters indicate significant differences (P ≤ 0.05) between treatment and the control-Botrytis.