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. 2020 Oct 28;14:591177. doi: 10.3389/fnins.2020.591177

FIGURE 4.

FIGURE 4

Inhibition of calcium signaling blocked the BHBA-induced increase of BDNF expression. (A,B) Western blot analysis of p-CaMKII (n = 3, F(3, 8) = 23.23) (A) and p-CREB (n = 3, F(3, 8) = 5.623) (B) levels in hippocampal neurons treated with 5 μM KN-62 for 10 min prior to treatment with 2 mM-BHBA treatment for another 30 min. (C) Western blot analysis of p-CREB levels in hippocampal neurons treated with 10 μM manidipine for 10 min or left untreated prior to treatment with 2 mM BHBA for 1 h (n = 4, F(3, 16) = 8.198). (D) Western blot analysis of BDNF expression in hippocampal neurons treated with 10 μM manidipine for 10 min or left untreated prior to treatment with 2 mM BHBA for 12 h (n = 4, F(3, 12) = 15.43). The data represent the mean ± SEM from three independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001.