Figure 4.

The ability of S. apiospermum to assimilate iron from xenosiderophores. (A, B) Colony growth phenotypes on PDA ± BPS after seven days of incubation at 37°C. (C, D) Ferrioxamine and ferrichrome (20 µM each) fully rescued the growth of all strains in the presence of 100 µM BPS. The fungus growing between Scedosporium colonies corresponds to contamination by Aspergillus sp. (E, F) Iron-bound pyoverdine (20 µM) fully restored growth for the WT and parent strains, while ΔsidD mutant strains only displayed oriented growth (arrows). Reddish pigmentation observed at the reverse of the colonies indicates the accumulation of iron. (G, H) Separated cultivation of WT, parent, and mutant strains, suggesting that N^α-methylcoprogen B is mandatory for iron acquisition from pyoverdine.