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. 2020 Oct 28;10:595466. doi: 10.3389/fonc.2020.595466

Figure 3.

Figure 3

Effects of radiotherapy and DHA on PD-L1, TGF-β, PI3K/Akt, and STAT3 signaling pathways in PC9 and PC9/X cells. (A) The MTT assay results of PC9 and PC9/X cell lines treated with vehicle, D, R, D+R. (B) The MTT assay results of PC9/X-NC and PC9/X-Si-PD-L1-RNA cell lines treated with vehicle, D, R, D+R. (C) The expressions of TGF-β, Akt, p-Akt, STAT3, p-STAT3, NF-κB, and PD-L1 in PC9 and PC9/X cell lines treated with vehicle, D, R, D+R by western blot. The values are expressed as means ± SEM. ap < 0.05 vs. the PC9 group, bp < 0.05 vs. the PC9/X group, cp < 0.05 vs. the PC9/X-R group; (D) The expressions of TGF-β, Akt, p-Akt, STAT3, p-STAT3, NF-κB, and PD-L1 in PC9/X transfected with NC or PD-L1 siNRA while treated with vehicle, D, R, D+R by western blot. The values are expressed as means ± SEM. ap < 0.05 vs. the PC9/X-NC group, bp < 0.05 vs. the PC9/X-Si-PD-L1-RNA group, cp < 0.05 vs. the PC9/X-Si-PD-L1-RNA+R group.