FIGURE 7.

PD-L2 influences AKT and ERK pathways. (A) Western blot analysis of pAKT (Ser473), pERK1/2(Thr202/Tyr204), ERK1/2, AKT, and GAPDH protein levels in PD-L2⁺ EC cells. (B) Western blot analysis of pAKT (Ser473), pERK1/2(Thr202/Tyr204), ERK1/2, AKT, and GAPDH protein levels in PD-L2^– EC cells. Blots are representative of one of three separate experiments. The pERK1/2(Thr202/Tyr204) and pAKT (Ser473) protein levels were determined with respect to ERK1/2 and AKT levels. ERK1/2 and AKT densitometry values were normalized to GAPDH used as loading control. Densitometric values shown are the mean ± SE of three separate experiments. *p < 0.05 vs control cells.