Figure 5.

AKAP1 was a target of miR-431 in hepatocellular cancer cells. (A) The binding sites between miR-431 and AKAP1 were predicted using the Starbase database. Dual-luciferase reporter assays were conducted for the evaluation of the luciferase activity in 293T cells contransfected with WT or MUT reporter vectors of AKAP1 and (B) miRNA NC, miR-431 mimic, (C) inhibitor NC or miR-431 inhibitor. Effect of miR-431 on expression of AKAP1 (D) mRNA and (E) protein of SNU-387 and Huh7 cells was analyzed by RT-qPCR and western blot analysis, respectively. Under hypoxic conditions, the (F) mRNA and (G) protein levels of AKAP1 in SNU-387 and Huh7 cells were detected by RT-qPCR and western blot analysis, respectively. *P<0.05. AKAP1, A-kinase anchor protein 1; miR, microRNA; WT, wild-type; MUT, mutant; NC, negative control; RT-qPCR, reverse transcription-quantitative PCR; UTR, untranslated region.