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. 2020 Aug 5;69(9):1169–1178. doi: 10.1099/jmm.0.001238

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© 2020 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.

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Fig. 1. — Limit of detection of N1-STOP-LAMP and establishing optimal sample template volume and inactivation conditions. (a) Plot showing performance of the N1 LAMP assay across 5-log₁₀ dilution of purified viral RNA. Y-axis is time-to-positive (Tp) and the x-axis is an estimate of viral genomes/reaction based on starting RNA concentration. The number of replicates per dilution (n) and number of positive replicates per dilution is indicated. (b) Example N1-STOP-LAMP amplification plots for SARS-CoV-2 positive clinical sample No. 52, showing inhibitory impact of 5 µl of a neat sample matrix on LAMP and the effect of diluting the UTM in water. (c) N1-STOP-LAMP amplification plots for three SARS-CoV-2 positive clinical samples, showing no loss in detection sensitivity after specimen heat-treatment of 60 °C for 30 min to inactivate virus.