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. 2020 Nov 10;10:19416. doi: 10.1038/s41598-020-76375-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of Nef Y120F and Q125H mutations on counteraction of SERINC3/5-mediated infectivity inhibition. (A) Infectivity of viruses that were produced from JTAg cells and JTAg-SERINC3/5^−/− cells (left panel). Those cells were transduced with HIV-1 NL43 proviral constructs lacking Nef (ΔNef) or carrying Nef_SF2 and the mutants (F121A and D123A), and then TZM-bl cells were exposed to the resultant viruses to determine viral infectivity. Results are expressed as the mean of triplicate assessments, normalized to the control strain, NL4.3-Nef_SF2. Statistical analysis was performed by using the paired t test. Ability of Nef_SF2 and the indicated mutants (F121A and D123A) to counteract SERINC3/5 was determined by dividing the relative infectivity of viral particles secreted from JTAg cells by that from JTAg-SERINC3/5^−/− cells (right panel). (B) Specific SERINC3/5 counteraction function of Nef_SF2 and the indicated mutants (Y120F, Q125H, Y120F/Q125H). Data shown are the mean results ± SD from 3 independent experiments. Statistical analysis was performed by ANOVA with multiple comparisons vs Nef_SF2. n.s. not significant.