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. 2020 Nov 10;10:19423. doi: 10.1038/s41598-020-76565-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Visible and FTIR images of LBs in the medullary dorsal vagal nucleus derived from the PD patients (PD1-4, PD2-1, PD3-2, and PD4-1). Shown from left to right are the microscopy images, the amounts of total protein, the proportions of the β-sheet structure, and the spectra at all points in the scan. The colour bar indicates low (blue) to high (red) contents. The area shaded in green in the visible image was scanned with 3 µm steps. 7 × 7 points = 21 × 21 µm². The red solid line in the spectra shows the FTIR spectrum of the β-sheet richest point.