Figure 1.

Generation of rat anti-human claudin-6 (CLDN6) monoclonal antibodies (mAbs). (A) Amino acid sequences of the antigenic peptide of the C-terminal cytoplasmic domains of human CLDN6 and the corresponding regions of the closely related CLDNs. Conserved amino acids are shown in red. (B) The constructs of CLDN1/4/5/6/9 expression vectors and the representative fluorescence images of the transfected HEK293T cells. EF-1a, elongation factor-1a; IRES, internal ribosome entry site. (C) Twenty-four hybridoma clones were screened by Western blotting for CLDN6 in HEK293T cells that were transiently transfected with the CLDN6 or empty expression vector. (D,E) HEK293T cells were transfected with individual CLDN expression vectors, and subjected to Western blotting and immunohistochemical analyses using the indicated anti-CLDN6 Abs. (F) The complementarity-determining regions (CDRs) of an anti-human CLDN6 mAb (clone #15). Scale bars, 100 µm.