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. 2020 Nov 10;10:19476. doi: 10.1038/s41598-020-75207-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Bacterial species clustering based on the abundance of each species (heat map) at different taxonomic rank in piglets samples in relation with ill-diarrhea and curing. Log-scaled percentage heat map of phylum- (A), class- (B), order- (C), family- (D), genus- (E) and species- (F) level. Longitudinal clustering indicates the similarity of all species among the four piglet samples. The horizontal clustering indicates the similarity of certain species among the four samples. The closer the distance is and the shorter the branch length is, the more similar the species composition is between the samples. At phylum, all species were used to draw the heatmap. The species of which abundance is less than 0.5% in all samples were classified into “others” in other ranks. The individual bacterial species are represented as colors (gplots, R(v3.1.1), BGI Co., Ltd).