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Primers used in vector construction and transgenic plant identification.
| Application | Primer name | Primer Sequence (5′–3′)a |
|---|---|---|
| RNAi template | R-Kpn I-F | GGGGTACCTCTGGAATTTGCTCGCGTTC |
| R-BamH I-R | CGGGATCCATACAGCTCTCAGGAAGCCC | |
| R-Spe I-F | GGACTAGTTCTGGAATTTGCTCGCGTTC | |
| R-Xba I-R | GCTCTAGAAAACCCTGACCTCGCAGTTA | |
| Hygromycin | Hyg-R | ACGGTGTCGTCCATCACAGTTTGCC |
| Identification | Hyg-F | TTCCGGAAGTGCTTGACATTGGGGA |
aRestriction site sequences are underlined.
