Table 2.

Constant process conditions during the optimization process of each single-factor experiment.

Optimizing condition	Constant process conditions
EClH concentration [% (v/v)]	pH 7.5, 400 rpm, 4 °C, 0.02 mg/mL ADH, 2 h immobilization time
Immobilization temperature (°C)	4% (v/v) EClH, pH 7.5, 400 rpm, 4 °C, 0.02 mg/mL ADH, 2 h immobilization time
Rotation speed (rpm)	4% (v/v) EClH, pH 7.5, 4 °C, 0.02 mg/mL ADH, 2 h immobilization time
pH of the medium (/)	4% (v/v) EClH, 500 rpm, 4 °C, enzyme concentration 0.02 mg/mL, 2 h immobilization time
Immobilization time (h)	4% (v/v) EClH, pH 7.5, 500 rpm, 4 °C, enzyme concentration 0.02 mg/mL
ADH concentration (mg/mL)	4% (v/v) EClH, pH 7.5, 500 rpm, 4 °C, 2 h immobilization time