Table 1.

Conditions and primers used for PCR, amplified product base pair size, and references used for PCR in this study

PCR assay	Name of primer seq (5′-3′)	PCR conditions				Product Size (bp)	Reference
		Denaturation	Annealing	Extension	Cycles
		(°C/sec)	(°C/sec)	(°C/sec)
16 s rRNA PCR for tick identification	16S - 1-F (CTGCTCAATGAATATTTAAATTGC)	95/45	55/60	72/90	40	450	(https://shire.science.uq.edu.au/parasites/arachnids/ticks/ticks-identification.php)
	16S-1-R (CGGTCTAAACTCAGATCATGTAGG)
ankA N-PCR for Anaplasma phagocytophilum	ANK-F1 (GAAGAAATTACAACTCCTGAAG)	95/30	53/30	72/60	35	705	[7]
	ANK-R1 (CAGCCAGATGCAGTAACGTG)
	ANK-F2 (TTGACCGCTGAAGCACTAAC)	95/30	52/30	72/60	5	664
	ANK-R2 (ACCATTTGCTTCTTGAGGAG)	95/30	54/30	72/60	25
groEL N-PCR for Anaplasma phagocytophilum	HS1 TGGGCTGGTA(A/C)TGAAAT	94/60	48/120	70/90	3	1300	[8]
	HS6 CCICCIGGIACIA(C/T)ACCTTC	88/60	52/120	70/90	37
	HS43 AT(A/T)GC(A/T)AA(G/A)GAAGCATAGTC	94/60	48/120	70/90	3	528
	HS45 ACTTCACG(C/T)(C/T)TCATAGAC	88/60	55/120	70/90	37
ompA N-PCR for Rickettsia spp.	R190.70F (ATGGCGAATATTTCTCCAAAAA)	94/30	50/30	72/60	40	634	[9]
	RR190.701R (GTTCCGTTAATGGCAGCATCT)
	R190.70F (ATGGCGAATATTTCTCCAAAAA)	94/30	51/30	72/30	5	535
	RR190.602R (AGTGCAGCATTCGCTCCCCCT)	94/30	55/30	72/30	30
56 kDa N-PCR for Orientia tsutsugamushi	56BO-144F (YGYAGAATCTRCTCGCTTGG)	94/60	60/60	72/60	35	1250	In this study
	56BO-1395R (agctaMccctRcaccaaBac)
	56BO-406F (CCWCCTCARCCTACTAtrTGC)	94/30	61/30	72/45	30	680
	56BO-1088R (gcWgctgctRctgcttcttg)
pyrG N-PCR for Borrelia spp.	pyrG-1F (ATTGCAAGTTCTGAGAATA)	94/20	45/30	72/30	30	801	[10]
	pyrg-1R (CAAACATTACGAGCAAATTC)
	pyrG-2F (GATATGGAAAATATTTTATTTATTG)	95/30	45/30	72/30	5	707
	pyrg-2R (AAACCAAGACAAATTCCAAG)	95/30	47/30	72/30	5
		95/30	49/30	72/30	25
RT-PCR for SFTSV	MF3 (GATGAGATGGTCCATGCTGATTCT)	95/20	58/40	72/30	35	560	[11]
	MR2 (CTCATGGGGTGGAATGTCCTCAC)