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. 2013 Sep 5;104(11):1544–1551. doi: 10.1111/cas.12248

Figure 4.

Figure 4

Ying Yang 1 (YY1) positively regulates heme oxygenase‐1 (HO‐1) in esophageal cancer cells. (a) TE‐1 cells were stably transfected with YY1 overexpression vector (pcDNA3.1‐YY1) or control vector (pcDNA3.1). YY1, HO‐1, P21 and GAPDH protein levels were detected by Western blot. (b) Cells were transfected with an shRNA targeting YY1 (shRNA‐YY1) or control shRNA (shRNA‐NC) for 24 h. HO‐1, YY1 and GAPDH protein levels were detected by Western blot. (c) A luciferase reporter containing the HO‐1 promoter was co‐transfected with indicated vectors or shRNAs into TE‐1 cells. After 24 h of incubation, luciferase activity was measured using the Dual‐Luciferase Reporter Assay System (Promega). TE‐1 cell lines were co‐transfected with indicated siRNA and vector, and evaluated for cell proliferation (e) and focus formation (f). *P < 0.05, compared with pcDNA3.1 tranfected cells.