Figure 8.

Immunofluorescence multiple staining with anti‐CD45, anti‐cytokeratin, anti‐green fluorescent protein (GFP) antibodies. The GFP positive cells in normal esophagus also showed CD45 positivity and identified as leukocyte. A few GFP positive cells did not show CD45 positivity. These cells also positive for cytokeratin and identified as epithelium cells derived from transplanted bone marrow cells. There was no GFP positive, CD45 negative and cytokeratin positive cells in Barrett's mucosa. A few cells in carcinoma showed GFP positivity, CD45 negativity and cytokeratin positivity.