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. 2020 Nov 11;51:135. doi: 10.1186/s13567-020-00859-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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The DEV UL47 gene is a late gene. A Total RNA isolated from mock-infected and DEV-infected cells at various times was analysed using RT-qPCR and normalized to β-actin, and plotted the fold change over mock. B The expression analysis of the UL47 gene product in DEV-infected DEFs at different time points. The samples derived from the same experiment and gels were processed in parallel. C Schematic diagram showed the IE gene (UL54), E gene (UL13), and L gene (US2). D GCV group: DEV-infected cells treated with GCV; CHX group: DEV-infected cells treated with CHX. (+) group: DEV-infected cells without drugs. M, DL2000 Marker.