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. 2020 Nov 11;51:135. doi: 10.1186/s13567-020-00859-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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The residues 40–50 and 768–777 of DEV pUL47 could direct heterologous proteins to the nucleus. All transfected samples were collected after 24 h posttransfection. A DEF cells were transfected with pEGFP-C2-SV40, pEGFP-C2-40-50, pEGFP-C2-768-777 and pEGFP-C2, respectively. B The expression of proteins was confirmed by Western blotting using mouse anti-GFP monoclonal antibody and goat anti-mouse IgG HRP-conjugated antibody (1:5000) (magnification: 200×; scale bar: 10 μm). C DEF cells were transfected with pEGFP-C2-SV40-β-gal, pEGFP-C2-40-50-β-gal, pEGFP-C2-768-777-β-gal and pEGFP-C2-β-gal, respectively. D The expression of proteins was confirmed by western blotting using mouse anti-GFP monoclonal antibody.