Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 11;51:135. doi: 10.1186/s13567-020-00859-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

pUL47 interacted with STAT1 in the cytoplasm and inhibited ISG expression induced by STAT1. A HEK293T cells were coexpressed pcaggs-STAT1-myc with pcaggs-UL47-Flag, pcaggs-STAT1-myc with an empty vector and pcaggs-UL47-Flag with an empty vector (magnification: 200×; scale bar: 10 μm). B BHK21 cells contained pcDNA3.1-C-UL47-LgBiT coexpressed with pcDNA3.1-N-STAT1-SmBiT, FKBP-SmBiT coexpressed with FRB-LgBiT as positive controls, Halotag-SmBiT coexpressed with pcDNA3.1-C-UL47-LgBiT, pcDNA3.1-C-UL47-LgBiT coexpressed with FKBP-SmBiT and pcDNA3.1-N-STAT1-SmBiT coexpressed with FRB-LgBiT as negative controls. The luciferase signal was detected via NanoBiT after 20-h posttransfection. C DEV UL47 inhibited the mRNA levels of Mx and OASL induced by STAT1. DEF cells were co-transfected with pcaggs-STAT1-myc and pcaggs-UL47-Flag or an empty vector. The cells were harvested and detected by RT-qPCR at 36 h posttransfection. Protein expression was confirmed by Western blotting. All experiments were performed in triplicate (n = 3). The data was analyzed by one-way ANOVA. *P < 0.05, **P < 0.01 and ****P < 0.0001.