Table 1.
Sequences and primer pair characteristics
| Primer | Plasmid | Primer sequence (5′ → 3′) |
|---|---|---|
| P1 | pET-32a-UL47 | G/GATCCAGACAGCGCCGTAGGTCA |
| P2 | C/TCGAGAACTATTGCCGGATTAACAGG | |
| P3 | DEV UL47 (RT-PCR) | AACGGAGTTGCTTGGAGAACA |
| P4 | TGGGCGATGAAACAGAGTAGG | |
| P5 | Duck β-actin (RT-PCR) | CCGGGCATCGCTGACA |
| P6 | GGATTCATCATACTCCTGCTTGCT | |
| P7 | DEV UL54 (RT-PCR) | GAACAACCGCCGAACAC |
| P8 | TCAAACATCCGCCTCAA | |
| P9 | DEV UL13 (RT-PCR) | GCCACCAACCCTACCAAG |
| P10 | GTCGTCAGCCCATCACCA | |
| P11 | DEV Us2 (RT-PCR) | AGACGGTTCCGAAAGTACAG |
| P12 | TCGGCAGCACCAATAATCC | |
| P13 | pcaggs-UL47-Flag | CATCATTTTGGCAAAG/AATTCGCCACCATGGCCATGGATAAATCACGAAGAC |
| P14 | TTGGCAGAGGGAAAAA/GATCTTTACTTGTCATCGTCGTCCTTGTAATCATGTAACTCTCTCCGCCC | |
| P15 | pcasggs-STAT1-myc | CATCATTTTGGCAAAG/AATTCGCCACCATGGCCGAGCAGAAACTCATCTCTGAAGAGGATCTGATGACTCAGTGGTACCAG |
| P16 | GGCAGAGGGAAAAA/GATCTTTAAGTTGAATATGCTGAACAC | |
| P17 | pEGFP-C2-40-50 | AATTCAGGAGAAGTGGTAAGAGACGTACACTTGACAGGG |
| P18 | GATCCCCTGTCAAGTGTACGTCTCTTACCACTTCTCCTG | |
| P19 | pEGFP-C2-768-777 | AATTCAAAGCATTAAAACGACGTTTGACTGGTGGGG |
| P20 | GATCCCCCACCAGTCAAACGTCGTTTTAATGCTTTG | |
| P21 | pEGFP-C2-40-50-β-gal | CTCGAGCTCAAGCTTCG/AATTCAGGAGAAGTGGTAAGAGACGTACACTTGACAGGATGAGCGAAAAATACATCG |
| P22 | CAGTTATCTAGATCCGGTG/GATCCTTATTTTTGACACCAGAC | |
| P23 | pEGFP-C2-768-777-β-gal | CTCGAGCTCAAGCTTCG/AATTCAAAGCATTAAAACGACGTTTGACTGGTGGGATGAGCGAAAAATACATCG |
| P24 | CAGTTATCTAGATCCGGTG/GATCCTTATTTTTGACACCAGAC | |
| P25 | pcDNA3.1 (+)-Flag-UL47Δ40-50-768-777 | GCTTGGTACCGAGCTCG/GATCCGCCACCATGGATTACAAGGATGACGACGATAAGATGGATAAATCACGAA |
| P26 | GTTTAAACGGGCCCTCTAGAC/TCGAGTTAATGTAACTCTCTCCGC | |
| P27 | pcDNA3.1-C-UL47-LgBiT | CTGTTGGTAAAGCCACCA/GATCTGCCACCATGGATAAATCACGAAG |
| P28 | CCGCTCCCGCCACCACCGC/TCGAGGGCTTGTCATCGTCGTCCTTGTAATCATGTAACTCTCTCCGCCC | |
| P29 | pcDNA3.1-N-STAT1-SmBiT | GAGCGGAGGTGGAGGC/TCGAGCATGACTCAGTGGTACCAG |
| P30 | CCGCCCCGACTCTAGAA/GATCTTTACAGATCCTCTTCAGAGATGAGTTTCTGCTCAGTTGAATATGCTGAACAC |