Fig. 3.

Heat treatment of mPER2 demonstrates that mPER2 has the biochemical characteristics of an IDP. To demonstrate that mPER2 remained soluble after heat stability, murine liver lysates were subjected to heat treatment (∆H: 100 °C for 10 min) with a mock treatment on ice in parallel. Centrifugation was used to separate the soluble fraction from the aggregated proteins. For western blot visualization, 10 μg of total protein was loaded per lane for all treatments, with the Santa Cruz Per-2 H-90 (sc-25,363) antibody used for detection