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. 2020 Jun 18;41(11):1427–1432. doi: 10.1038/s41401-020-0452-0

Fig. 3. Early procedures for electroporation, puromycin selection, and analysis of UCHL-1 expression by immunoblotting.

Fig. 3

a Representative bright-field (upper panels) and GFP (lower panels) microscope images at the indicated time points after electroporation. b Single colonies of edited cells were analyzed by immunoblotting for the expression of UCHL-1. Ponceau red staining was used to indicate equal loading.