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. Author manuscript; available in PMC: 2020 Nov 11.
Published in final edited form as: Neuroreport. 2008 Apr 16;19(6):687–690. doi: 10.1097/WNR.0b013e3282fbcef7

Fig. 1.

Fig. 1

Differentiated PCI2 chromatin immunoprecipitation (ChIP)-derived DNA was used as template for PCR reactions designed to amplify a fragment of the rat β4 promoter. Amplification was observed for both histone H4 (lane 2) and input (lane 8) ChIP positive controls. The expected PCR product was also produced in Sp1, Sp3 and c-Jun reactions (lanes 4–6). No amplification was observed when using DNA immunoprecipitated by normal mouse IgG (lane 3), no antibody (lane 7) or in no-template PCR reactions (lane 9). The arrow indicates the 200-base pair marker (lane 1). Each ChIP experiment was carried out a minimum of three times.