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. Author manuscript; available in PMC: 2020 Nov 11.
Published in final edited form as: Neuroreport. 2008 Apr 16;19(6):687–690. doi: 10.1097/WNR.0b013e3282fbcef7

Fig. 3.

Fig. 3

OBL21 chromatin immunoprecipitation (ChIP)-derived DNA was used as a template for PCR reactions designed to amplify a 243-base pair product of the mouse β4 promoter.The expected product was observed in PCR reactions for both histone H4 (lane 2) and input (lane 9) positive controls. Amplification was also observed in Sp1, Sp3, Sox10 and c-Jun immunoprecipitations (lanes 4–7). No amplification was seen in PCR reactions using DNA immunoprecipitated by normal mouse IgG (lane 2), no antibody (lane 8) or in no-template PCR reactions (lane 10). The arrow indicates the 250-base pair marker (lane 1). Each ChIP experiment was carried out a minimum of three times.