Table 3.

Effect of CA and CDCA on expression of genes involved in lipid metabolism and inflammation in the liver.

Role	Gene	WD	WD-CA	WD-CDCA	One way Anova
		n=6	n=6	n= 6	P
Cholesterol homeostasis	Cyp7a1	1.04 ± 0.32	0.37 ± 0.18**	0.38 ± 0.28**	0.0006
	Cyp3a11	1.01 ± 0.19	0.48 ± 0.17***	0.48 ± 0.12***	0.0001
	Cyp8b1	1.01 ± 0.17	0.08 ± 0.04***	0.65 ± 0.18	0.0008
	Hmgcr	1.06 ± 0.40	0.13 ± 0.06**	0.62 ± 0.36	0.0051
	Srebf2 (SREBP2)	1.01 ± 0.16	0.27 ± 0.04***	0.68 ± 0.19**	0.0001
	Ldlr	1.07 ± 0.39	0.38 ± 0.10*	0.94 ± 0.25	0.0153
BAs signaling	Slco1a1 (OATP1)	1.01 ± 0.15	0.91 ± 0.34	1.19 ± 0.29	NS
	Slc10a1 (NTCP)	1.10 ± 0.52	0.44 ± 0.25	2.07 ± 0.71*	0.0003
	Slc51b (OSTb)	1.09 ± 0.50	6.46 ± 2.61**	1.26 ± 0.69*	0.0047
	Abcb11 (BSEP)	1.01 ± 0.12	0.85 ± 0.19	1.11 ± 0.21	NS
	Fgf4	1.00 ± 0.12	0.79 ± 0.13*	0.96 ± 1.14	0.0287
Cholesterol transport	Abca1	1.02 ± 0.20	1.50 ± 0.21***	1.43 ± 0.10**	0.0005
	Nr1h3 (LXRα)	1.01 ± 0.13	0.73 ± 0.15**	0.93 ± 0.08	0.0034
	Abcg1	1.02 ± 0.23	3.06 ± 0.41***	1.52 ± 0.22*	0.0001
	Abcg5	1.03 ± 0.24	2.27 ± 0.71*	1.50 ± 0.19*	0.013
	Abcg8	1.03 ± 0.29	2.37 ± 0.66***	1.75 ± 0.40*	0.0007
	Scarb1 (SR-B1)	1.02 ± 0.22	1.20 ± 0.22	1.09 ± 0.21	NS
	Cav-1	1.01 ± 0.15	0.50 ± 0.31**	0.58 ± 0.21*	0.0034
Nuclear receptors	Nr1i2 (PXR)	1.01 ± 0.19	0.80 ± 0.26	1.09 ± 0.17	NS
	Nr1h4 (FXR)	1.06 ± 0.35	0.79 ± 0.27	1.39 ± 0.31	0.0146
	Nr0b2 (SHP)	1.31 ± 1.29	3.68 ± 1.07**	2.97 ± 0.89*	0.0059
	Vdr	1.09 ± 0.45	5.70 ± 3.06**	1.44 ± 0.50	0.0008
	Rxrα	1.02 ± 0.22	0.77 ± 0.23	1.19 ± 0.27	0.0302
Fatty acids and TG biosynthesis	Pparγ	1.04 ± 0.32	0.31 ± 0.06**	0.88 ± 0.17	0.0024
	Fasn	1.02 ± 0.24	0.25 ± 0.08***	0.88 ± 0.36	0.0085
	Dgat1	1.01 ± 0.14	0.67 ± 0.15**	1.03 ± 0.12	0.0005
	Srebf1 (SREBP-1c)	1.05 ± 0.35	1.54 ± 0.57	2.33 ± 0.39***	0.0007
Inflammation	Tnfα	1.06 ± 0.38	2.34 ± 0.80**	0.94 ± 0.34	0.0008
	Il6	1.13 ± 0.60	0.53 ± 0.30	0.73 ± 0.36	NS

Results are presented as means ± SD.

For statistical significance, *P < 0.05, **P < 0.01, ***P < 0.001 vs. WD.

NS not significant.