Fig. 1.

A) Multiple sequence alignment of partial sequence of the nucleocapsid gene of MERS-CoV (NC 038294.1), SARS-CoV-2 (NC_045512.2), SARS-CoV (NC_004718.3) showing target regions of N1 and N2 primer/probe sets. Forward primers sequences are bolded, probes are faded and itilicized, and reverse primers are underlined and bolded. Note: 780 bases between the two primer sets were omitted to shorted the length of the sequence. B) Capilary gel electrophoresis picture generated using the Agilent DNA 7500 kit on the Agilent 2100 bioanalyzer instrument. Image showed single band for each primer set. From left to right: L (ladder:100 – 7000bp), N1, N2, and RP of aproximately 72 bp, 67 bp, and 65 bp, respectively.