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. 2020 Nov 11;133:104688. doi: 10.1016/j.jcv.2020.104688

Fig. 2.

Fig. 2

Real time PCR determining amplification efficiency of the primer/probe sets. Ten-fold serial dilution of 200,000 copies/μl of nCoVPC plasmid was tested. PCR linearity over 6 orders of magnitude with a limit of detection of 2 copies/μl; N1 slope of -3.05 with a correlation coefficient R2 = 0.99; N2 slope =-3.33 and R2 = 0.99.