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. 2020 Mar 20;58(8):1149–1161. doi: 10.1093/mmy/myaa013

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© The Author(s) 2020. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — In vitro induced capsule thickness and cell diameter of individual Cryptococcus neoformans strains from Vietnam: Cells were grown on DMEM medium/5% CO₂ and visually assessed by India ink staining. Images were taken for single cells measurement using ImageJ software. Capsule thickness is obtained by subtracting cell body diameter from total cell diameter. AFLP-VNI-γ/MLST-ST5 strains expressed higher degree of variation in both capsule size and cell diameter in vitro, which remains significant even when the outlier BMD1646 was removed from the analysis (P < .0001 for both capsule and cell size, Fligner-Killeen test). Scattered plot represents single cells from an individual strain. Data for individual strains are presented as mean with error bars denoting standard deviation. Strains selected for experiment in mice were indicated by asterisks.