Figure 2.

Growth factors enhance prostasphere formation in vitro. (A) Prostasphere formation was increased by hepatocyte growth factor (HGF). Prostasphere formation was performed in serum‐free Dulbecco's modified eagle medium (DMEM)/F12 media. Growth factors were added to the prostasphere culture. Representative pictures of prostaspheres are shown. Magnification, ×100. (B) Representative pictures of prostaspheres formed by ALDH1^high cells and ALDH1^low cells. ALDH1^high cells and ALDH1^low cells were cultured with or without HGF and IGF1. Representative pictures are shown. Magnification ×100. (C) Prostasphere formation of ALDH1^high cells and ALDH1^low cells with or without growth factors (HGF and IGF1). ALDH1^high cells and ALDH1^low cells were cultured in serum‐free media with or without HGF and IGF1. The numbers of prostaspheres were evaluated at day 3. Asterisks represents statistically significant difference (P < 0.05, t‐test).