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. 2012 Nov 8;104(1):48–54. doi: 10.1111/cas.12030

Figure 3.

Figure 3

MiR‐150 induces MET‐like changes in the E‐cadherin‐suppressed ESCC cell line TE‐8. (A) Immunocytochemical staining indicated E‐cadherin and vimentin expression in premiR‐150‐treated TE‐8 cells compared to the premiR‐nc‐treated cells. (B) Left panel: representative images of wound healing assays of premiR‐150‐treated TE‐8 cells. TE‐8 cells transfected with premiR‐nc or premiR‐150 were wounded (time zero) and maintained for 24 h in RPMI1640 with 10% FBS. Right panel: this figure shows the ratio of wound closure in TE‐8 cells transfected with premiR‐nc or premiR‐150. The error bars represents the SD from eight replicates. (C) Proliferation assay: the proliferation rate of premiR‐150‐treated cells was suppressed in comparison with that of premiR‐nc‐treated cells. The data represent the means ± SD (*P < 0.05).