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. 2013 Jan 13;104(2):178–184. doi: 10.1111/cas.12067

Figure 1.

Figure 1

(a) Ataxia telangiectasia mutated (ATM) immunoprecipitant contains a 170‐kDa protein. ATM was immunoprecipitated from unirradiated (−) and irradiated (IR) (+) wild‐type lymphoblastoid cell lines (WT) and ATR65RM cells (AT). Immunoprecipitants were visualized by Coomassie Brilliant Blue staining. The band at approximately 170 kDa (arrow) was subjected to peptide sequencing. SM, size marker. (b) Separation of ATM and topoisomerase II alpha (TOP2A) by sucrose density gradient (1, lightest fraction [Fr.]; 9, heaviest fraction). (c) Left panel, WT lymphoblastoid cell line (WT) and AT65RM (AT) cell lysates were immunoprecipitated (IP) with anti‐ATM or anti‐TOP2A antibody, and immunoprecipitants were immunoblotted (IB) with anti‐ATM or anti‐TOP2A antibody, respectively. Whole‐cell extract (WCE) was included as a size control. Right panel, cell lysates from 293T cells were immunoprecipitated with anti‐ATM or anti‐TOP2A antibody, with control IgG.