Figure 6.

Centrosomal localization of NuMA is affected by Nup188 depletion. (a) Subcellular localization of NuMA in Nup188‐depleted cells. HeLa cells were treated with mock or Nup188 siRNA for 48 h and with MG132 for the final 2 h. Cells were stained for NuMA (green), tubulin (red) and DNA (blue). Bar, 10 μm. (b) Nup188 interacts with NuMA. Total cell lysates prepared from HeLa cells treated with nocodazole were subjected to immunoprecipitation (IP) with anti‐Nup188 (upper) or anti‐NuMA (bottom) antibody. Lysates and IP were separated using SDS‐PAGE and probed using western blotting with anti‐Nup188 or anti‐NuMA antibody.