Figure 2.

In vitro coculture assay of lymphoma cell lines and human macrophages. (a) To analyze BrdU incorporation into cells, double‐immunostaining of BrdU (green) and CD204 (brown, a macrophage marker) was carried out. (b) ATN‐1 cells were cocultured with M1 or M2 macrophages derived from four healthy donors, and BrdU incorporation was analyzed. *Statistically significant (P < 0.05). (c) TL‐Mor cells were cocultured with M1 or M2 macrophages derived from four healthy donors, and BrdU incorporation was analyzed. (d) Lymphoma cell lines and M2 macrophages were directly cocultured with or without use of Transwell culture dishes, and BrdU incorporation was analyzed. Data were analyzed using Student's t‐test (n = 3–4).