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. 2013 May 9;104(7):945–951. doi: 10.1111/cas.12167

Figure 4.

Figure 4

Increased CD163 expression in macrophages as a result of direct cell−cell interaction. (a) Macrophage colony‐stimulating factor (M‐CSF)‐primed macrophages or granulocyte macrophage‐colony stimulating factor (GM‐CSF)‐primed macrophages were stimulated with interleukin (IL)‐4, IL‐10, tumor cell supernatant (TCS) of ATN‐1, and TCS of TL‐Mor for 2 days. CD163 expression was examined using Western blot analysis. IFN, interferon. (b) After M‐CSF‐primed macrophages (Mac) or GM‐CSF‐primed macrophages were cocultured with ATN‐1 cells indirectly or directly for 2 days, CD163 expression was examined using Western blotting. (c) CD163 expression in macrophages stimulated with TCS of ATN‐1 or cultured directly with ATN‐1 cells was evaluated using cell‐ELISA methods. Data were analyzed using Student's t‐test (n = 4).