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Identification of the binding motif(s) that mediate(s) the FRS2β‐CIN85 or CD2AP interaction. (A) Alignment of the ‘PxPxxR’ CIN85 or CD2AP binding motifs in FRS2β amino acid sequences of different species. (B) Schematic representation of the constructed FRS2β mutants. (C) Immuno‐precipitation of lysates of HEK293T cells expressing FLAG‐tagged FRS2β or its mutants and analysis of the precipitates and the whole cell lysate by Western blotting.
