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. 2013 Feb 8;104(3):345–352. doi: 10.1111/cas.12086

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© 2012 Japanese Cancer Association

PMC Copyright notice

FRS2β^‐eGFP and ^mC ^herry‐CIN85 co‐localized in T98G cells and expression of FRS2β inhibited anchorage‐independent cell growth. (A–C) ^mC ^herry‐CIN85 (A) and FRS2β^‐ ^eGFP (B) showed a punctuate expression pattern and frequent colocalization (C). (D–F) ^mC ^herry‐CIN85 showed a punctate expression pattern (D), whereas the RwA^‐ ^eGFP mutant exhibited a diffuse, non‐punctate, intracellular localization (E) and no colocalization with ^mC ^herry‐CIN85 (F). (G–I) Soft agar colony formation assay. Photographs were taken after 3 weeks. (H) Quantification of the number of colonies in soft agar after 3 weeks. Arrows indicate the colonies with a diameter >100 μm. (I) Cell lysates were immunoblotted with the indicated antibodies.