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. 2013 Mar 24;104(5):552–557. doi: 10.1111/cas.12128

Figure 2.

Figure 2

Suppression of KIF14 inhibits hepatocellular carcinoma (HCC)cells proliferation and migration in vitro. (a, b) HepG2 and SMMC‐7721 cells stably expressing KIF14 siRNA (RNAi‐15 and RNAi‐22; RNAi‐3 and RNAi‐11) or non‐sense siRNA (control) were lysed and cells were analyzed for KIF14 by RT‐qPCR and western blot, respectively. (c, d) Cell cycle distribution of KIF14 siRNA and controls serum starved for 12 h and then re‐stimulated with 10% FBS‐containing medium for the indicated time. (e, f) Cells were cultured in 96‐well plates and analyzed by MTT assay. Cell proliferation curves were shown in 9 days. Three independent experiments were conducted. (g, h) Cells were cultured in 6‐well plates and analyzed by colony formation assay. After 2 weeks, cells were stained and counted. Three independent experiments were conducted. (i, j) Cell migration motility was examined in 24‐well plates with Transwell chambers. After addition of 24 h incubation, cells adherent to the upper surface of the filter were removed using a cotton applicator. Cells migrated into the lower chambers were fixed with 70% methanol, stained with crystal violet. *P < 0.05.