Figure 5.

Suppression of MG132‐mediated ASK1 activation by 14‐3‐3 proteins. (A) U87 and SF295 cells were transfected with 14‐3‐3ε or 14‐3‐3θ/τ alone or in combination for 24 h, and then treated with MG132 for an additional 24 h, and Western blot was performed using the indicated antibodies. (B) U87 cells were transfected with the indicated 14‐3‐3 vectors for 24 h, and then treated with MG132 for an additional 24 h, and Western blot was performed using the indicated antibodies.