Figure 8.

Examination of IGF‐1 effects on SI NEN cell lines. A dose dependent effect on proliferation was evident with EC₅₀~7–100 pg/mL recombinant IGF‐1 (a). Effects of IGF‐1 (1000 pg/mL) on pIGF‐1R in each of the cell lines after 1 h treatment demonstrated upregulation of IGFR‐mediated signaling compared to untreated cells while pretreatment for 1 h with BMS‐754807 (10 μM) reversed phosphorylation (b). Pre‐treatment with BMS‐754807 reduced viability in both cell lines (c,d) and had both cytotoxic and apoptotic effects (estimated IC₅₀ = 2 × 10⁻⁷ M). Mean ± SEM, n = 6 (three separate experiments). CON, control (unstimulated); IGF, IGF‐1 stimulated; BMS, BMS‐754807 treated.