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. 2013 Mar 20;104(5):604–610. doi: 10.1111/cas.12120

Figure 4.

Figure 4

Effects of norcantharidin (NCTD) on Ki67 staining of tumor cells and CD34 staining of the blood vessels in non‐cancerous tissues in vivo. (a) Norcantharidin inhibits the proliferation of tumor cells in vivo measured by Ki67 immunostaining of the tumor tissues. The average percent positive Ki67 cells in control animals was 37.41 ± 2.12% (magnification, 200×), whereas the average percent positive Ki67 cells in animals treated with NCTD at dosage of 0.5, 1 and 2 mg/kg per day were 30.08 ± 3.3%, 22.24 ± 3.97%, and 19.72 ± 1.54%, respectively; percent positive Ki67 cells in 5‐Fu treated animals were 13.11 ± 3.04%. Column, mean; bar, SE (n = 6, *P < 0.05, **P < 0.01, ***P < 0.001 versus control). (b) Effects of NCTD on CD34 staining of the blood vessels in non‐cancerous tissues in vivo. The organs were fixed with 4% paraformaldehyde and embedded with paraffin. The 5‐μm sections were stained with anti‐mouse CD34 antibody. There was no difference of blood vessels in heart, kidney and lung between the control and the NCTD‐treat animals at dosage of 2 mg/kg per day.