Skip to main content
. 2013 Mar 20;104(5):604–610. doi: 10.1111/cas.12120

Figure 5.

Figure 5

Norcantharidin (NCTD) inhibits vascular endothelial growth factor (VEGF)‐induced activation of the phosphorylation of VEGFR2/MEK/ERK signaling pathway. (a) Effects of NCTD on the phosphorylation and activation of VEGFR2/MEK/ERK pathway. Human umbilical vein endothelial cells (HUVECs) were pretreated with various concentrations of NCTD for 10 min before stimulation with 50 ng/mL VEGF for a certain time. After that, cells were washed with cold phosphate‐buffered saline (PBS) and lysed on the dish in RIPA buffer. Phosphorylation and activation of different protein kinases including pTyr1175VEGFR2, pSer217/221‐MEK1/2, and pThr202/Tyr204‐pERK1/2 were examined by specific antibodies. (b,c) A time course of VEGF's effect on HUVECs without or with the presence of NCTD (40 μM): 0, 5, 10, 30, 60, 120 min. (d) Norcantharidin could not affect the phosphorylation of pThr180/Tyr182‐p38 MAPK, pSer473‐Akt and Cox‐2 expression levels.