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. 2013 May 19;104(8):1097–1106. doi: 10.1111/cas.12181

Figure 1.

Figure 1

(On the next page) Abnormal expression of Helios mRNA in primary adult T‐cell leukemia (ATL) cells. (a) Expression analysis of Ikaros family genes in PBMCs by full‐length RT‐PCR (Acute, n = 22; Chronic, n = 10; Smoldering, n = 5; HTLV‐1 carriers, n = 5; High‐risk carriers, n = 4). To detect and distinguish alternative splicing variants, PCR analyses were carried out with the sense and antisense primer sets designed in the first and final exons of each full‐length transcript of Ikaros family genes. Obtained cDNAs were cloned and their sequences were analyzed. The samples acute #4, 4', and 4'' were derived from the same patient, but were studied independently. (b) Expression of Ikaros family genes in PBMCs from normal volunteers (n = 10). (c) Schematic representation of Hel‐1, Hel‐2, and ATL‐type Helios isoforms identified in this study. Hel‐variant 1 (Hel‐v1) and Hel‐variant 2 (Hel‐v2) are novel isoforms in ATL. Arrows indicate primer locations of full‐length PCR for Helios. Ex, exon; F1–F6, functional zinc‐finger domains. (d) Nested PCR with specific primer sets, which were designed at exon junction of exon 1–5 or exon 2–5 for detection of Hel‐5 and Hel‐6 (upper panel), or detection of Hel‐v1 (lower panel), respectively. Arrows indicate primer locations.